Application

Detect Apolipoprotein A-II using this Anti-Apolipoprotein A-II Antibody validated for use in ELISA, IP, WB, IH(P).

Research CategoryMetabolism

Research Sub CategoryLipid Metabolism & Weight Regulation

Immunoblotting: 1:5,000-1:10,000

Immunohistochemistry on paraffin embedded tissue: 1:50-1:200

Immunoprecipitation

ELISA: 1:4,000-1:8,000 using 1 µg of Apo A-II in standard sandwich.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Apolipoprotein A-II, synthetic.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in 0.125 M Sodium Borate buffer, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, with 0.01% sodium azide.

Specificity

Apolipoprotein A-II, Human. Negligible cross reactivity to Apo A-I, B, C-I, C-II, C-III, E and J by ELISA. Negligible non-specific cross reaction with other human serum proteins.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for several weeks. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for up to 12 months. Avoid repeated freeze/thaw cycles.